Ubiquitin ELISA Kit

Catalog No. ABIN8088707

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse, Rat, Rabbit, Dog, Cow, Pig and Horse Ubiquitin.

Quick Overview for Ubiquitin ELISA Kit (ABIN8088707)

Target: Ubiquitin (Ub)

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Pig, Horse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6.25 ng/mL - 400 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details Ubiquitin ELISA Kit

Minimum Detection Limit: 6.25 ng/mL

Purpose: Human/Mouse/Rat/Rabbit/Dog/Pig/Bovine/Horse Ubiquitin (Ub) ELISA Kit for detecting Ub in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 2.42 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Gene Aliases: Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway