Vitamin D3 ELISA Kit

Catalog No. ABIN8089057

Various Species Vitamin D3 ELISA Kit, Colorimetric assay for quantification of Various Species Vitamin D3.

Quick Overview for Vitamin D3 ELISA Kit (ABIN8089057)

Target: Vitamin D3

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 4.94 ng/mL - 400 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Vitamin D3 ELISA Kit

Minimum Detection Limit: 4.94 ng/mL

Purpose: Vitamin D3 (VD3) ELISA Kit for detecting VD3 in serum, plasma

Analytical Method: Quantitative

Sensitivity: 2.14 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Vitamin D3

Target: Vitamin D3

Target Type: Chemical

Background: Gene Aliases: Cholecalciferol, Toxiferol, Activated 7-Dehydrocholesterol