ZAP70 ELISA Kit

Catalog No. ABIN8089227

The Human, Mouse and Rat ZAP70 ELISA Kit (ABIN8089227) is a Colorimetric ELISA Kit designed to quantify Human, Mouse and Rat ZAP70.

Quick Overview for ZAP70 ELISA Kit (ABIN8089227)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Binding Specificity: pTyr493

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details ZAP70 ELISA Kit

Purpose: Phospho-ZAP-70 (Tyr493) Colorimetric Cell-Based ELISA Kit for detecting Phospho-ZAP-70 (Tyr493) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP-70

Background: Gene Aliases: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway