Anti-Tumor Necrosis Factor alpha Antibody (TNFalpha Ab) ELISA Kit

Catalog No. ABIN8091902

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Anti-Tumor Necrosis Factor alpha Antibody.

Quick Overview for Anti-Tumor Necrosis Factor alpha Antibody (TNFalpha Ab) ELISA Kit (ABIN8091902)

Target: Anti-Tumor Necrosis Factor alpha Antibody (TNFalpha Ab)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Detection Range: 1.563 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details

Minimum Detection Limit: 1.563 ng/mL

Purpose: Human anti-TNF-α Antibody (Anti Tumor Necrosis Factor Alpha Antibody) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with anti-TNF-α Antibody , no obvious cross reaction with other analogues.

Sensitivity: 0.938 ng/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• HRP Antibody(Concentrated
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Indirect ELISA

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

Target Details

Target: Anti-Tumor Necrosis Factor alpha Antibody (TNFalpha Ab)

Alternative Name: TNF-alpha Antibody

Target Type: Antibody

Background: TNF-α antibody, Tumor Necrosis Factor Alpha antibody

UniProt: P01375