CXCL9 ELISA Kit

Catalog No. ABIN8126235

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CXCL9.

Quick Overview for CXCL9 ELISA Kit (ABIN8126235)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details CXCL9 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensitivity: 94 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human CXCL9 concentrations in serum, plasma. Detection Range: 156 - 10,000 pg/mL Sensitivity: <94 pg/mL

Application Details

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human CXCL9 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human CXCL9 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CXCL9 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human CXCL9 can be calculated using the standard curve.  

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: CXCL9/MIG (C-X-C motif chemokine 9)