Interferon gamma ELISA Kit

Catalog No. ABIN8126237

Human Interferon gamma ELISA Kit, Colorimetric assay for quantification of Human Interferon gamma.

Quick Overview for Interferon gamma ELISA Kit (ABIN8126237)

Target: Interferon gamma (IFNG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human IF-gamma antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IFN-gamma detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IFN-gamma amount of samples captured in the plate.,This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensitivity: 9.4 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human IFN-gamma concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL  

Application Details

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human IF-gamma antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IFN-gamma detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IFN-gamma amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IFN-gamma can be calculated using the standard curve. 

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-Gamma (Interferon Gamma)

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy