Osteopontin ELISA Kit

Catalog No. ABIN8126238

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Osteopontin.

Quick Overview for Osteopontin ELISA Kit (ABIN8126238)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Osteopontin ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 93.75 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human OPN concentrations in serum, plasma.  Detection Range: 0.156 - 10 ng/mL Sensitivity: < 93.75 pg/mL

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate. Standards and samples are pipetted into the wells and OPN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human OPN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of OPN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: OPN (Osteopontin)

Pathways: Regulation of Cell Size