TIMP4 ELISA Kit

Catalog No. ABIN8126239

The Human TIMP4 ELISA Kit (ABIN8126239) is a Colorimetric ELISA Kit designed to quantify Human TIMP4.

Quick Overview for TIMP4 ELISA Kit (ABIN8126239)

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details TIMP4 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: This assay employs an antibody specific for Anti-human TIMP-4 coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 46.875 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human TIMP-4 concentrations in serum, plasma. Detection Range: 78 - 5000 pg/mL Sensitivity: < 46.875 pg/mL

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human TIMP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIMP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for TIMP4

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Alternative Name: TIMP-4 (Metalloproteinase Inhibitor-4)