APOE ELISA Kit

Catalog No. ABIN8126242

The Human APOE ELISA Kit (ABIN8126242) is a Colorimetric ELISA Kit designed to quantify Human APOE.

Quick Overview for APOE ELISA Kit (ABIN8126242)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 23.44 ng/mL - 1500 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details APOE ELISA Kit

Minimum Detection Limit: 23.44 ng/mL

Purpose: This assay employs an antibody specific for Anti-human APOE  coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 14.063 ng/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human APOE concentrations in serum, plasma.  This kit is for Research Use Only. Not for use in diagnostic/therapeutics procedures. Detection Range: 23.44 - 1500 ng/mL Sensitivity: < 14.063 ng/mL

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human APOE  coated on a 96-well plate. Standards and samples are pipetted into the wells and APOE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human APOE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of APOE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE (Apolipoprotein E)

Pathways: Regulation of Cell Size, Lipid Metabolism