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IL-10 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-10.
Catalog No. ABIN8126244
$740.00
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 5 to 6 Business Days

Quick Overview for IL-10 ELISA Kit (ABIN8126244)

Target

See all IL-10 (IL10) ELISA Kits
IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity

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Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6 pg/mL - 1000 pg/mL

Application

ELISA

Sample Type

Plasma, Serum
  • Minimum Detection Limit

    15.6 pg/mL

    Purpose

    This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate.

    Analytical Method

    Quantitative

    Characteristics

    This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-10 concentrations in serum, plasma.  Detection Range: 15.6 - 1000 pg/mL Sensitivity: <9.4pg / ml
  • Plate

    Pre-coated

    Protocol

    This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

    Restrictions

    For Research Use only
  • Storage Comment

    Please refer to the Manual
  • Target See all IL-10 (IL10) ELISA Kits

    IL-10 (IL10) (Interleukin 10 (IL10))

    Alternative Name

    IL-10 (Interleukin 10)

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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