IL-10 ELISA Kit
Quick Overview for IL-10 ELISA Kit (ABIN8126244)
Target
See all IL-10 (IL10) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 15.6 pg/mL
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Purpose
- This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate.
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Analytical Method
- Quantitative
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Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-10 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: <9.4pg / ml
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Plate
- Pre-coated
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Protocol
- This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Restrictions
- For Research Use only
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Storage Comment
- Please refer to the Manual
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- IL-10 (IL10) (Interleukin 10 (IL10))
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Alternative Name
- IL-10 (Interleukin 10)
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
Target See all IL-10 (IL10) ELISA Kits
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