IL1A ELISA Kit

Catalog No. ABIN8126246

Mouse IL1A ELISA Kit, Colorimetric assay for quantification of Mouse IL1A.

Quick Overview for IL1A ELISA Kit (ABIN8126246)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL1A ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate.

Analytical Method: Quantitative

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-1a concentrations in serum, plasma.  Detection Range: 15.6- 1000 pg/mL Sensitivity: < 9.4 pg / ml

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1 Alpha (Interleukin-1 Alpha)

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints