IL23 ELISA Kit

Catalog No. ABIN8126248

The Mouse IL23 ELISA Kit (ABIN8126248) is a Colorimetric ELISA Kit designed to quantify Mouse IL23.

Quick Overview for IL23 ELISA Kit (ABIN8126248)

Target: IL23 (Interleukin 23 (IL23))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL23 ELISA Kit

Purpose: This assay employs an antibody specific for Anti-mouse IL-23 coated on a 96-well plate.

Analytical Method: Quantitative

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-23 concentrations in serum, plasma.    Detection Range: 15.6- 1000 pg/mL   Sensitivity: < 9.4 pg / ml    

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-mouse IL-23 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for IL23

Target: IL23 (Interleukin 23 (IL23))

Alternative Name: IL-23 (Interleukin 23)