IL-33 ELISA Kit

Catalog No. ABIN8126249

Mouse IL-33 ELISA Kit, Colorimetric assay for quantification of Mouse IL-33.

Quick Overview for IL-33 ELISA Kit (ABIN8126249)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-33 ELISA Kit

Minimum Detection Limit: 15.625 pg/mL

Purpose: This assay employs an antibody specific for Anti-mouse IL-33 coated on a 96-well plate.

Analytical Method: Quantitative

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-33 concentrations in serum, plasma.  Detection Range: 15.625-1000pg/mL Sensitivity: <9.375pg/mL  

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-mouse IL-33 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-33 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-33 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-33 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33 (Interleukin 33)

Pathways: Production of Molecular Mediator of Immune Response