CXCL2 ELISA Kit

Catalog No. ABIN921067

Mouse CXCL2 ELISA Kit Colorimetric assay for quantification of Mouse CXCL2 and has been mentioned in 3+ publications.

Quick Overview for CXCL2 ELISA Kit (ABIN921067)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Binding Specificity: AA 28-100

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details CXCL2 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MIP-2

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: A28-N100

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <5pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A28-N100

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MIP-2 has been precoated onto 96-well plates. Standards(E.coli, A28-N100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MIP-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse MIP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MIP-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 113, Standard deviation: 4.41, CV(%): 3.9
• Sample 2: n=16, Mean(pg/ml): 355, Standard deviation: 17.75, CV(%): 5
• Sample 3: n=16, Mean(pg/ml): 627, Standard deviation: 40.13, CV(%): 6.4,
• Sample 1: n=24, Mean(pg/ml): 134, Standard deviation: 8.31, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 387, Standard deviation: 21.3, CV(%): 5.5
• Sample 3: n=24, Mean(pg/ml): 653, Standard deviation: 44.4, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for CXCL2 ELISA Kit (ABIN921067)

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Roux, Schaefers, Clark, Weatherholt, Renaud, Scott, LiPuma, Priebe, Gerard, Yoder-Himes: "A putative lateral flagella of the cystic fibrosis pathogen Burkholderia dolosa regulates swimming motility and host cytokine production." in: PLoS ONE, Vol. 13, Issue 1, pp. e0189810, (2018) (PubMed).

Roux, Weatherholt, Clark, Gadjeva, Renaud, Scott, Skurnik, Priebe, Pier, Gerard, Yoder-Himes: "Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa." in: Infection and immunity, Vol. 85, Issue 6, (2017) (PubMed).

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: CXCL2

Background:

Protein Function: Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.

Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein(MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein(MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water(aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.

Synonyms: C-X-C motif chemokine 2,Macrophage inflammatory protein 2,MIP2,Cxcl2,Mip-2, Mip2, Scyb2,

Full Gene Name: C-X-C motif chemokine 2

Cellular Localisation: Secreted.

Gene ID: 20310

UniProt: P10889

Pathways: Cellular Response to Molecule of Bacterial Origin