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PLG ELISA Kit

PLG Reactivity: Human AA 98-355 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN921122
  • Target See all PLG ELISA Kits
    PLG (Plasminogen (PLG))
    Binding Specificity
    AA 98-355
    Reactivity
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Angiostatin K1-3
    Brand
    PicoKine™
    Sample Type
    Serum, Plasma, Cell Culture Supernatant
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO,V98-S355(K1-3)
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: V98-S355(K1-3)
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.

    Tissue Specificity: Present in plasma and many other extracellular fluids. It is synthesized in the liver.

    Plate
    Pre-coated
    Protocol
    human Angiostatin K1-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiostatin K1-3 has been precoated onto 96-well plates. Standards (NSO,V98-S355(K1-3)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiostatin K1-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiostatin K1-3 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiostatin K1-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human serum, plasma or cell culture supernates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Angiostatin K1-3 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.09, Standard deviation: 0.041, CV(%): 3.8
    • Sample 2: n=16, Mean(ng/ml): 3.68, Standard deviation: 0.155, CV(%): 4.2
    • Sample 3: n=16, Mean(ng/ml): 6.96, Standard deviation: 0.320, CV(%): 4.6,
    • Sample 1: n=24, Mean(ng/ml): 1.13, Standard deviation: 0.057, CV(%): 5
    • Sample 2: n=24, Mean(ng/ml): 3.75, Standard deviation: 0.199, CV(%): 5.3
    • Sample 3: n=24, Mean(ng/ml): 7.04, Standard deviation: 0.415, CV(%): 5.9
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all PLG ELISA Kits
    PLG (Plasminogen (PLG))
    Alternative Name
    PLG (PLG Products)
    Synonyms
    wu:fb70e09 ELISA Kit, PLG ELISA Kit, LPA ELISA Kit, plg ELISA Kit, Ab1-346 ELISA Kit, AI649309 ELISA Kit, Pg ELISA Kit, plasminogen ELISA Kit, plg ELISA Kit, PLG ELISA Kit, Plg ELISA Kit
    Background

    Protein Function: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. .

    Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(EDâ,...â,€ =70 nM) relative to kringles 1-4(EDâ,...â,€ = 135 nM).

    Synonyms: Plasminogen,3.4.21.7,Plasmin heavy chain A,Activation peptide,Angiostatin,Plasmin heavy chain A, short form,Plasmin light chain B,PLG,

    Full Gene Name: Plasminogen

    Cellular Localisation: Secreted . Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
    Gene ID
    5340
    UniProt
    P00747
    Pathways
    Complement System, Lipid Metabolism
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