IGF1 ELISA Kit

Catalog No. ABIN930359

Product Details IGF1 ELISA Kit

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Method Type: Sandwich ELISA

Application: ELISA

Characteristics: ELISA kit for the detection of Human IGF1 in the research laboratory
Alternative Names: IGF1 ELISA kit, Insulin-like Growth Factor 1 ELISA kit, Human IGF1 ELISA kit, IGF-1 ELISA kit

Purification: Immunoaffinity chromatography

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This ELISA for IGFI E20 is a socalled SandwichAssay using two specific and high affinity antibodies. The IGFI in the samples binds to the first antibody coated on the microtiterplate, the second specific antiIGFIantibody binds inturn to the immobilised IGFI. The second antibody is biotinylated, the subsequently incubated StreptavidinperoxidaseEnzyme Conjugate will bind to it, and thus in the final substrate incubation step colour development will be catalysed quantitatively depending on the IGFIlevel of the samples. The Standards of the ELISA E20 are preparedfrom recombinant IGFI in concentrations of 1, 5, 15, 30 and 50 ng/mL.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1 (IGF1 Products)

Background: Insulin-like growth factors (IGF) I and II play a pivotal role in regulating the proliferation, differentiation and specific functions of many cell types. IGFI is identical with Somatomedin C (SmC) and has a molecular weight of 7649 Da. Its major regulators are growth hormone (GH) and nutrition, although its production in specific tissues is affected by a multitude of tropic hormones and other peptide growth factors. In contrast to many other peptide hormones, IGFs are avidly bound to specific binding proteins (IGFBP). The seven classes of IGFBPs which are known at present either bind IGFI and IGFII with similar affinities or show a preference for IGFII.
Synonyms: IGF1 ELISA kit, Insulin-like Growth Factor 1 ELISA kit.

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation