IGF1 ELISA Kit

Catalog No. ABIN411285

The Rat IGF1 ELISA Kit (ABIN411285) is a Colorimetric ELISA Kit designed to quantify Rat IGF1. This ELISA Kit has been cited in 12 publications.

Quick Overview for IGF1 ELISA Kit (ABIN411285)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Binding Specificity: AA 49-118

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details IGF1 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich Rat IGF-1 ELISA Kit to quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: G49-A118

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat specific for IGF-1

Cross-Reactivity (Details): There is detectable cross-reactivity with IGF-2 < 1 % .

Sensitivity: < 5 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.4%, 7.5%, 4.3%
Inter-Assay Precision (CV%): 6%, 9.1%, 5.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for IGF1 ELISA Kit (ABIN411285)

Yang, Wu, Feng, Huang, Liu, Liu, Chen: "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 267, (2017) (PubMed).

Ozdemir, Aksu, Baykara, Ates, Sisman, Kiray, Buyuk, Uysal: "Effects of administration of subtoxic doses of acetaminophen on liver and blood levels of insulin-like growth factor-1 in rats." in: Toxicology and industrial health, Vol. 32, Issue 1, pp. 39-46, (2016) (PubMed).

Cai, Tan, Zhang, Li, Wang, Zhu, Wang, Yang, Wang, Liu, Xu, Pan, Wang, Yang, Lu: "Mesenchymal Stem Cells and Cardiomyocytes Interplay to Prevent Myocardial Hypertrophy." in: Stem cells translational medicine, Vol. 4, Issue 12, pp. 1425-35, (2016) (PubMed).

Hou, Wan, Wang, Li, Wang, Yao, Feng, Jing, Lu, Jia, Peng: "Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression." in: American journal of translational research, Vol. 8, Issue 9, pp. 3656-3665, (2016) (PubMed).

Niu, Kou, Zhou, Ding: "Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats." in: Neural regeneration research, Vol. 7, Issue 21, pp. 1611-7, (2015) (PubMed).

Chen, Yang, He, Song, Liu: "Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis." in: Neural regeneration research, Vol. 8, Issue 19, pp. 1756-64, (2014) (PubMed).

Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

Cai, Li, Wang, Liu, Yang, Chen, Yin, Tan, Zhu, Pan, Wang, Lu: "Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via activating JNK signal." in: PLoS ONE, Vol. 8, Issue 5, pp. e63561, (2013) (PubMed).

Aksu, Baykara, Kiray, Gurpinar, Sisman, Ekerbicer, Tas, Gokdemir-Yazar, Uysal: "Serum IGF-1 levels correlate negatively to liver damage in diabetic rats." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 3-4, pp. 194-201, (2013) (PubMed).

Ni, Sun, Fu, Wang, Guo, Tian, Wei: "IGF-1 promotes the development and cytotoxic activity of human NK cells." in: Nature communications, Vol. 4, pp. 1479, (2013) (PubMed).

Baykara, Aksu, Buyuk, Kiray, Sisman, Baykara, Dayi, Tas, Ozdemir, Arda, Uysal et al.: "Progesterone treatment decreases traumatic brain injury induced anxiety and is correlated with increased serum IGF-1 levels; prefrontal cortex, amygdala, hippocampus neuron density; and reduced serum ..." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 5, pp. 250-7, (2013) (PubMed).

Iseli, Hedinger: "Histopathology and ultrastructure of primary adrenocortical nodular dysplasia with Cushing's syndrome." in: Histopathology, Vol. 9, Issue 11, pp. 1171-94, (1986) (PubMed).

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1

Background:

Background: Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98 % of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Gene Full Name: insulin-like growth factor 1

Gene ID: 24482

UniProt: P08025

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation