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DNA Oxidative Damage - Quantitative Kit

Colorimetric
Catalog No. ABIN955769
  • Target
    Oxidative DNA Damage
    Detection Method
    Colorimetric
  • Restrictions
    For Research Use only
  • Target
    Oxidative DNA Damage
    Alternative Name
    Oxidative DNA Quantitative
    Background
    The Oxidative DNA Damage Kit, Quantitative is for the determination of abasic sites in sample DNA, which correspond to colorimetric 96-well microplate assay. ARP standard DNA solutions in this kit are prepared by heat / acid depurination of calf thymus DNA to control the number of abasic sites from 0 to 40 per 1x10 5 bp. These DNA solutions are treated with ARP and purified. The Standard 0 ARP-DNA is prepared using methoxyamine-treated calf thymus DNA without heat/ acid depurination. Since this kit contains centrifugal filtration tubes for the purification of ARP-labeled sample DNA, there is no need to determine the DNA concentration after the ARP reaction. Standard ARP DNA and purified ARP-treated sample DNA are fixed on the 96 well plate with DNA Binding Solution. Then, the number of AP sites in the sample DNA can be determined by the biotin-avidin-peroxidase assay. Since the standard ARP DNA are double GENERAL.Purification of genomic DNA: Several different methods and products are available for the isolation of genomic DNA from samples such as membrane binding method, guanidine/detergent lysis method, and polyelectrolyte precipitation method. Among these methods, the guanidine/detergent lysis method is simple, and it gives highly purified genomic DNA for the ARP-based abasic sites detection.
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