DNA Quantification Kit

Catalog No. ABIN955791

Quick Overview for DNA Quantification Kit (ABIN955791)

Target: DNA Quantification

Detection Method: Fluorometric

Product Details

Components: Color Developer (H33258): 10 mL x 1
Buffer: 125 mL x 2
Calibrator (DNA 100 µg/mL): 2 mL x 1
Kit Size: Enough reagent for 200 samples.

Material not included: Adjustable pipettor
Fluorometer
Purified water

Application Details

Reagent Preparation:

Serial dilute the stock DNA Calibrator (100 µg/mL) with purified water to 50, 25, and 12.5 µg/mL. Use the purified water for a 0 µg/mL calibrator and the DNA calibrator stock for 100 µg/mL. Diluted assay calibrators can be stored frozen at -20°C. Do not thaw the diluted calibrators more than 1 time.

Calculation of Results:

1. Centrifuge cultured cells.
   2. Remove supernatant and resuspend the cell pellet in buffer.
   3. Sonicate cells until cells are homogenized.
   4. Add 1 mL of buffer to 50 µL of cell homogenate and 1 mL of buffer to each assay calibrator.
   5. Mix to evenly distribute the sample in the buffer.
   6. Add 50 µL of Color Developer to each tube.
   7. Mix thoroughly.
   8. Measure fluorescence (excitation at wavelength 356 nm, emission at 458 nm).

Restrictions: For Research Use only

Handling

Handling Advice: 1. Read the instructions carefully before beginning the assay.
2. This kit is for research use only, not for human or diagnostic use.
3. Great care has been taken to ensure the quality and reliability of this product. However, it is possible that in certain cases, unusual results may be obtained due to high levels of interfering factors.

Storage: 4 °C

Storage Comment: Kit components can be stored at 4°C until expiration date.

Expiry Date: The expiry date is stated on the label.

Target Details

Target: DNA Quantification

Background: Reagent H33258 binds with DNA and forms a fluorescent substance (emission=485 nm). By adding the H33258 directly to a cell homogenate, DNA quantification can be performed without purifying DNA. This kit contains the same buffer which is used for ' S other cell function-measuring kits (Acidic Mucopolysaccharide Assay, TRAP Staining, and GPDH Assay). Therefore, the same sample can be shared among those assays and the DNA Quantification Kit. For research use only, not for diagnostic use.