Gelsolin ELISA Kit

Catalog No. ABIN955872

Product Details Gelsolin ELISA Kit

Target: Gelsolin (GSN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 ng/mL

Minimum Detection Limit: 3.9 ng/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human GS in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of human GS. No significant cross- reactivity or interference between human GS and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between human GS and all the other analogues, therefore, cross reaction may still exist.

Sensitivity: The minimum detectable dose of human GS is typically less than 1.5 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.

Characteristics: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GS. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of GS in the samples is then determined by comparing the O.D. of the samples to the calibration curve.

Components: Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x).

Material not included: 1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Comment:

The calibration curve concentrations used for the ELISA's were 250 ng/mL, 125 ng/mL, 62.5 ng/mL, 31.2 ng/mL, 15.6 ng/mL, 7.8 ng/mL, 3.9 ng/mL.

Plate: Pre-coated

Calculation of Results:

Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with GS concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.

Expiry Date: The expiry date is stated on the label.

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: Gelsolin (GS) (GSN Products)

Synonyms: ADF ELISA Kit, AGEL ELISA Kit, CG1106 ELISA Kit, DGS ELISA Kit, Dmel\\CG1106 ELISA Kit, gel ELISA Kit, scin ELISA Kit, cb107 ELISA Kit, gsn ELISA Kit, sb:cb107 ELISA Kit, u-gelsolin ELISA Kit, wu:fi16f06 ELISA Kit, gelsolin ELISA Kit, Gelsolin ELISA Kit, gelsolin S homeolog ELISA Kit, gelsolin a ELISA Kit, GSN ELISA Kit, Gel ELISA Kit, Gsn ELISA Kit, gsn.S ELISA Kit, gsn ELISA Kit, gsna ELISA Kit

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy