LDL ELISA Kit

Catalog No. ABIN955928

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat LDL.

Quick Overview for LDL ELISA Kit (ABIN955928)

Target: LDL (Low Density Lipoprotein (LDL))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 123.5-10000 ng/mL

Application: ELISA

Product Details LDL ELISA Kit

Minimum Detection Limit: 123.5 ng/mL

Purpose: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of rat LDL in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of rat LDL. No significant cross- reactivity or interference between rat LDL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between rat LDL and all the analogues, therefore, cross reaction may still exist.

Sensitivity: The minimum detectable dose of rat LDL is typically less than 47.2 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.

Characteristics: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for rat LDL has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled rat LDL and unlabeled rat LDL (Calibrators or samples) with the pre-coated antibody specific for rat LDL. After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of LDL in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LDL in the sample.

Components: Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A 1 x 120 µL
Assay Diluent A (2X concentrate) 1 x 6 mL
TMB Substrate 1 x 9 mL
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x).

Material not included: 1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Comment:

The calibration curve concentrations used for the ELISA's were 10, 000 ng/mL, 3, 333.3 ng/mL, 1111.1 ng/mL, 370.4 ng/mL, 123.5 ng/mL.

Plate: Pre-coated

Calculation of Results:

This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between LDL concentration in the sample and the assay signal intensity. Low levels of LDL result in a high OD value, while high concentration of LDL results in a low signal. Average the duplicate readings for each calibrator, control, and samples. Create a calibration curve on log-log graph paper, with LDL concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.

Expiry Date: The expiry date is stated on the label.

Target Details for LDL

Target: LDL (Low Density Lipoprotein (LDL))

Alternative Name: LDL