dsDNA Ab ELISA Kit

Catalog No. ABIN996874

Product Details dsDNA Ab ELISA Kit

Target: dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Purpose: dsDNA ELISA is intended for the detection of antibodies in human serum to dsDNA antigen and as an aid in diagnosis of Systemic lupus eruthematosus (SLE).

Sample Type: Serum

Analytical Method: Semi-Quantitative

Specificity: 97.9 %

Sensitivity: 100%

Components: 1. Microplate: wells coated with Purified dsDNA antigen
2. Serum Diluent Type II
3. Positive Control: Human serum or defibrinated plasma
4. Calibrator: Human serum or defibrinated plasma
5. Negative Control: Human serum or defibrinated plasma
6. HRP Conjugate: Goat anti-human IgG and IgM
7. Wash Buffer Type I 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: 1N H2SO4 solution

Material not included: 1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a (single wavelength 450 nm or dual 600-650)

Application Details

Application Notes: Notable features of Autoimmune Disease ELISA Kits:
- User-friendly directions and explanation of test procedures
- Simple and safe reagent preparation
- Clear instructions on specimen collection
- Comprehensive package of required materials
- Explicit quality control and storage guidelines
- Reliable and easy-to-read test results

Sample Volume: 10 μL

Assay Time: 1.5 h

Plate: Pre-coated

Protocol: dsDNA elisa test is an Enzyme-Linked Immunosorbent Assay to detect antibodies to dsDNA antigen. Purified dsDNA antigen is attached to the wells. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen in the wells forming antigen-antibody complexes. Excess antibody is removed by washing and followed by the addition of goat anti-human IgG, M conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12-14 months

References for dsDNA Ab ELISA Kit (ABIN996874)

Salazar, Copeland, Wood, Schmid, Luebke: "Evaluation of anti-nuclear antibodies and kidney pathology in Lewis rats following exposure to Libby amphibole asbestos." in: Journal of immunotoxicology, (2012) (PubMed).

Target Details for dsDNA Ab

Target: dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))

Alternative Name: DsDNA Antibody (dsDNA Ab Products)

Target Type: Antibody, Antibody

Background: Autoimmune diseases are categorized into two general groups: One group is characterized by the production of tissue-specific autoantibodies, the other by autoimmune reactivity with normal cell nuclear and/or cytoplasmic antigens with no tissue type specificity. The latter group includes diseases such as mixed connective tissue disease, scleroderma, rheumatoid arthritis, dermatomyositis, polymyositis, SLE and Sjogren syndrome.