PRTN3 ELISA Kit

Catalog No. ABIN996889

PRTN3 ELISA Kit, Colorimetric assay for quantification of PRTN3.

Quick Overview for PRTN3 ELISA Kit (ABIN996889)

Target: PRTN3 (Proteinase 3 (PRTN3))

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Product Details PRTN3 ELISA Kit

Purpose: Proteinase-3 (PR-3) Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the detection and semiquantitative determination of antibodies to PR-3 in human sera. The assay is to be used to detect antibodies in a single serum specimen. The results of the assay are to be used as an aid in the diagnosis of Wegener’s granulomatosis

Analytical Method: Semi-Quantitative

Specificity: 95.6%

Sensitivity: 98.2%

Application Details

Sample Volume: 10 μL

Assay Time: 1 - 2 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Alternative Name: PR3 (c-ANCA)

Background: Proteinase-3 is a lysosomal enzyme that can be found in human neutrophils. There are two subsets of autoantibodies to human neutrophils: c- ANCA and p-ANCA. The first subtype, c-ANCA, shows cytoplasmic staining by IFA and is diagnostic for Wegener's granulomatosis. The major antigen responsible for the c-ANCA staining is Proteinase-3. The second subtype, p-ANCA, shows perinuclear staining by IFA. The major antigen responsible for the p-ANCA response has been shown to be myeloperoxidase. The ANCA staining patterns are obtained using ethanol-fixed human neutrophils. Anti- PR-3 autoantibodies have been associated with Wegener's granulomatosis but less often with microscopic polyangiitis. Other antigen specificity's (CAP 57) can be responsible for the c-ANCA pattern. These antigens are generally not diagnostic for PR-3 associated vaculitus diseases.