Leishmania IgG ELISA Kit

Catalog No. ABIN997045

Human Leishmania IgG ELISA Kit, Colorimetric assay for quantification of Human Leishmania IgG.

Quick Overview for Leishmania IgG ELISA Kit (ABIN997045)

Target: Leishmania IgG

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Serum

Product Details

Purpose: The Leishmania infantum IgG ELISA is intended for the qualitative determination of IgG class antibodies against Leishmania infantum in human serum or plasma (citrate, heparin).

Analytical Method: Qualitative

Specificity: 92.59%

Sensitivity: 90.91%

Application Details

Sample Volume: 10 μL

Assay Time: 1 - 2 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: Leishmania IgG

Target Type: Species

Background: Visceral Leishmaniasis (VL) is a severe disease with high mortality, caused by parasite members of the L. donovani complex. The vector for transmission is the sand fly, whose carriers of infection are typically dogs. It is a disease endemic to many countries and is a serious problem in many developing nations, particularly with the increasing urbanization of populations. High incidence is encountered in parts of Latin America, East Africa, Middle East, India and China. It is endemic to countries bordering the Mediterranean such as Italy, Southern France, Spain, Portugal, and Northern Africa. In Southern Europe, VL has become the leading opportunistic infection in AIDS patients. Diagnosis of acute VL is often attempted by aspiration of bone marrow for direct parasite identification. The procedure is invasive, painful, dangerous and has a low success rate due to the inability to always isolate parasites from the tissue. Alternatively, serodiagnosis is widely utilized since anti-leishmanial antibody titers are typically high during the acute disease phase. ELISA is the preferred laboratory test for serodiagnosis of VL, although indirect immunofluorescent antibody tests (IFAT) and direct agglutination tests (DAT), using whole parasites, are still widely used in conjunction with ELISA or alone.