Cryptosporidium ELISA Kit

Catalog No. ABIN997059

The Cryptosporidium parvum Cryptosporidium ELISA Kit (ABIN997059) is a Colorimetric ELISA Kit designed to quantify Cryptosporidium parvum Cryptosporidium. This ELISA Kit has been cited in 1 publication.

Quick Overview for Cryptosporidium ELISA Kit (ABIN997059)

Target: Cryptosporidium

Reactivity: Cryptosporidium parvum

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details

Purpose: This ELISA is an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces.

Analytical Method: Qualitative

Specificity: 100 %

Application Details

Sample Volume: 1 g

Assay Time: 1 - 2 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

References

Nassar, Oyekale, Oluremi: "Prevalence of Cryptosporidium infection and related risk factors in children in Awo and Iragberi, Nigeria." in: Journal of immunoassay & immunochemistry, (2016) (PubMed).

Target Details

Target: Cryptosporidium

Target Type: Parasite

Background: Cryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men.

Diagnosis of Cryptosporidium infections was done originally by direct detection techniques. Of these, microscopic examination of stools using stains or fluorescence labeled antibodies has been the most common. However, this method relies on an experienced technician and subsequent observation of intact organisms. Because of the historically low proficiency of correct microscopic examinations, alternative diagnostic methods have been investigated. One important alternative has been the development of an antigen capture enzyme linked immunosorbent assay (ELISA) for use with stools. These tests, which have shown comparable sensitivity to experienced microscopic examinations, are fairly simple to perform and do not require the observation of intact organisms.