Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Lhca2 antibody

Lhca2 Reactivity: Arabidopsis thaliana, Barley, Green bean, Moss, Nicotiana tabacum, Oryza sativa, Pisum sativum WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5706927
  • Target
    Lhca2 (psi Type II Chloropyll A/B-Binding Protein (Lhca2))
    Reactivity
    Arabidopsis thaliana, Barley, Green bean, Moss, Nicotiana tabacum, Oryza sativa, Pisum sativum
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 23 / 24 kDa for Arabidopsis thaliana
    Purification
    affinity purified
    Immunogen
    BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana UniProt: Q9SYW8, Q8LCQ4, TAIR: At3g61470. This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At3g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.
    Isotype
    IgG
  • Application Notes
    Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).
    Comment

    A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA freepeptide, indicating that all bands are indeed PsbA and its precursors or breakdown product.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µL of sterile water
    Buffer
    PBS pH 7.4
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
  • Scotti, Sannino, Idoine, Hamman, De Stradis, Giorio, Maréchal-Drouard, Bock, Cardi: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, (2014) (PubMed).

    Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

    Yamatani, Sato, Masuda, Kato, Morita, Fukunaga, Nagamura, Nishimura, Sakamoto, Tanaka, Kusaba: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).

  • Target
    Lhca2 (psi Type II Chloropyll A/B-Binding Protein (Lhca2))
    Background
    AGI Code: At3g61470
    The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.
    Molecular Weight
    expected: 23 kDa, apparent: 24 kDa for Arabidopsis thaliana
    UniProt
    Q8LCQ4, Q9SYW8
You are here:
Support