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NADH Dehydrogenase Protein (His tag)

Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN5505730
  • Target
    NADH Dehydrogenase
    Protein Type
    Recombinant
    Origin
    Human
    Source
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This NADH Dehydrogenase protein is labelled with His tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human Purified recombinant protein of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22 kDa (NDUFB10), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag) protein expressed in E.coli.
    • Produced with end-sequenced ORF clone
    Purification
    Purified
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the N-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    NADH Dehydrogenase
    Background
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProtKB/Swiss-Prot Function]
    Molecular Weight
    20.6 kDa
    NCBI Accession
    NP_004539
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