PAB1 antibody
PAB1
Reactivity: Mammalian, Saccharomyces cerevisiae
ICC, IF, IHC, WB
Host: Mouse
Monoclonal
1G1
unconjugated
(2 references)-
- Target
- PAB1 (Pab1p (PAB1))
- Reactivity
- Mammalian, Saccharomyces cerevisiae
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- conc. tissue culture supernatant
- Clone
- 1G1
- Isotype
- IgG2a
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- Application Notes
- For immunofluorescence use MCA-1G1 diluted 1/500. For western blots try MCA-1G1 at dilutions of 1/1,000 to 1/5,000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4°C short term or -20°C long term.
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: "Surveying the global landscape of post-transcriptional regulators." in: Nature structural & molecular biology, Vol. 30, Issue 6, pp. 740-752, (2023) (PubMed).
: "Biochemical approach for isolation of polyadenylated RNAs with bound proteins from yeast." in: STAR protocols, Vol. 2, Issue 4, pp. 100929, (2022) (PubMed).
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: "Surveying the global landscape of post-transcriptional regulators." in: Nature structural & molecular biology, Vol. 30, Issue 6, pp. 740-752, (2023) (PubMed).
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- Target
- PAB1 (Pab1p (PAB1))
- Alternative Name
- Pab1p
- Synonyms
- polyadenylate-binding protein antibody, PAB1 antibody
- Background
- Pab1p is a protein which binds poly-A tails of yeast mRNA molecules, and is known as polyadenylate binding protein. It is also referred to as PAB1, ACBP, CST11 and MRNPand the systematic name is YER165w. It is they only yeast poly-A binding protein, and is found in both the cytoplasm and nucleus of yeast cells. The protein has a molecular weight of 64 kDa, and runs on typical SDS-PAGE gels at 67 kDa.
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