(4 references)-
- Target See all TNF alpha products
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Reactivity
- Mouse
- Application
- ELISpot
- Brand
- BD™ ELISPOT
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin level is ≤0.01 ng/μg of protein.
- Components
- This product contains sufficient reagent for five 96-well plates, including unlabelled capture antibody (no azide/low endotoxin format), biotinylated detection antibody, and a Certificate of Analysis that provides lot-specific optimal reagent concentrations.
-
TNF (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Mouse
TNF (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Mouse
TNF (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Mouse
-
- Application Notes
- The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
- Comment
-
BD™ ELISPOT Mouse TNF ELISPOT Pair
- Restrictions
- For Research Use only
-
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm filtered.
- Preservative
- Without preservative
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
-
-
: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).
: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).
: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." in: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).
: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).
-
: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).
-
- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- TNF (TNF alpha Products)
- Synonyms
- DIF Matched Antibody Pair, TNF-alpha Matched Antibody Pair, TNFA Matched Antibody Pair, TNFSF2 Matched Antibody Pair, RATTNF Matched Antibody Pair, Tnfa Matched Antibody Pair, tnf Matched Antibody Pair, TNF-a Matched Antibody Pair, TNFalpha Matched Antibody Pair, Tnfsf1a Matched Antibody Pair, TNFa Matched Antibody Pair, cTNF Matched Antibody Pair, Tnf-alpha Matched Antibody Pair, tnfa-like Matched Antibody Pair, TNF-ALPHA Matched Antibody Pair, dif Matched Antibody Pair, tnfa Matched Antibody Pair, xtnf Matched Antibody Pair, tnfsf2 Matched Antibody Pair, tnf-alpha Matched Antibody Pair, Cachectin Matched Antibody Pair, tumor necrosis factor Matched Antibody Pair, tumor necrosis factor b (TNF superfamily, member 2) Matched Antibody Pair, tumor necrosis factor alpha Matched Antibody Pair, tumor necrosis factor a (TNF superfamily, member 2) Matched Antibody Pair, TNF Matched Antibody Pair, Tnf Matched Antibody Pair, tnf Matched Antibody Pair, tnfb Matched Antibody Pair, tnf-alpha Matched Antibody Pair, LOC103694380 Matched Antibody Pair, tnfa Matched Antibody Pair
-
You are here:
