Human FGF21 EZ Set™ ELISA Kit (DIY Antibody Pairs)
-
- Target See all FGF21 products
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- Binding Specificity
- AA 29-209
- Reactivity
- Human
- Detection Method
- Colorimetric
-
Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects FGF21 in Human samples.
- Brand
- EZ-Set™
- Specificity
-
Expression system for standard: E.coli
Immunogen sequence: H29-S209 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Characteristics
-
Sequence similarities: Belongs to the heparin-binding growth factors family.
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
-
1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
-
FGF21 (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
FGF21 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
FGF21 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
FGF21 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
FGF21 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit
FGF21 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
FGF21 (Human) IP-WB Antibody PairIP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
-
- Comment
-
The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
-
- Buffer
-
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
-
- Target
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- Alternative Name
- FGF21 (FGF21 Products)
- Synonyms
- FGF21 Matched Antibody Pair, fibroblast growth factor 21 Matched Antibody Pair, FGF21 Matched Antibody Pair, fgf21 Matched Antibody Pair, Fgf21 Matched Antibody Pair
- Background
-
Protein Function: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. .
Background: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al.(2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.
Synonyms: Fibroblast growth factor 21,FGF-21,FGF21,UNQ3115/PRO10196,
Full Gene Name: Fibroblast growth factor 21
Cellular Localisation: Secreted. - UniProt
- Q9NSA1
-
