Maternal Embryonic Leucine Zipper Kinase (MELK) (N-Term) Peptide
MELK
Reactivity: Human, Mouse
Host: Synthetic
BP, WB
Catalog No. ABIN2184957
Quick Overview for Maternal Embryonic Leucine Zipper Kinase (MELK) (N-Term) Peptide (ABIN2184957)
Target
MELK
(Maternal Embryonic Leucine Zipper Kinase (MELK))
Origin
Human, Mouse
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- N-Term
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Sequence
- GFAKVKLACH VLTGEMVAIK IMDKNALGSD LPRVKTEIDA LKSLRHQHIC
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-Melk Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Maternal Embryonic Leucine Zipper Kinase (MELK) peptide
MELK Reactivity: Human, Mouse, Cow, Dog Host: Synthetic BP, Imm
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))
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Background
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Melk is a Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity, phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Melk Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It Plays a key role in cell proliferation. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. It's also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus and may also play a role in primitive hematopoiesis.
Alias Symbols: AI327312, MPK38, mKIAA0175
Protein Size: 643 -
Molecular Weight
- 70 kDa
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Gene ID
- 17279
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NCBI Accession
- NM_010790, NP_034920
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UniProt
- Q61846
Target
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