Tyrosyl-DNA Phosphodiesterase 1 (TDP1) (C-Term) Peptide
TDP1
Reactivity: Mouse, Human
Host: Synthetic
BP, WB
Catalog No. ABIN2185631
Quick Overview for Tyrosyl-DNA Phosphodiesterase 1 (TDP1) (C-Term) Peptide (ABIN2185631)
Target
TDP1
(Tyrosyl-DNA Phosphodiesterase 1 (TDP1))
Origin
Mouse, Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- C-Term
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Sequence
- GRPPGKSAVP LHLIYPSVEN VRTSLEGYPA GGSLPYSIQT AEKQRWLHSY
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-Tdp1 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))
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Background
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Tdp1 is a DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. It catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue and Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Tdp1 acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. It Has low h3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups and has no exonuclease activity towards DNA or RNA with a 3'phosphate.
Protein Size: 609 -
Molecular Weight
- 66 kDa
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UniProt
- Q8BJ37
Target
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