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Thyroid Peroxidase (TPO) (C-Term) peptide (Biotin)

TPO Reactivity: Human Host: Synthetic BP, Imm
Catalog No. ABIN5539407
  • Target See all Thyroperoxidase (TPO) products
    Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))
    Protein Region
    C-Term
    Origin
    Human
    Source
    • 5
    Synthetic
    Purification tag / Conjugate
    This Thyroperoxidase peptide is labelled with Biotin.
    Application
    Blocking Peptide (BP), Immunogen (Imm)
    Sequence
    ELRCGKHQAV GTSPQ.
    Predicted Reactivity
    Human
  • Application Notes
    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 110 kDa band observed in Human Thyroid Gland lysates (calculated MW of 103 kDa according to NP_000538.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
    Buffer
    100µg of Lyophilised peptide, formulation De-ionized (DI) water.
    Storage
    -20 °C
    Storage Comment
    Shipped at ambient temperature, store at -20°C.
  • Target
    Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))
    Synonyms
    MSA Peptide, TDH2A Peptide, TPX Peptide, TPO Peptide, msa Peptide, tpx Peptide, xtpo Peptide, LOC100218312 Peptide, thyroid peroxidase Peptide, TPO Peptide, Tpo Peptide, tpo Peptide, LOC100547103 Peptide
    Gene ID
    7173
    NCBI Accession
    NP_000538, NP_783650, NP_783652, NP_783653
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