3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1) (AA 321-332), (Internal Region) Peptide
HMGCS1
Reactivity: Human
Host: Synthetic
BP, Imm
Catalog No. ABIN5539419
$251.71
Plus shipping costs $50.00
100 μg
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Quick Overview for 3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1) (AA 321-332), (Internal Region) Peptide (ABIN5539419)
Target
HMGCS1
(3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- AA 321-332, Internal Region
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Sequence
- KASSELFSQK TK
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Predicted Reactivity
- Human
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Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 57 kDa band observed in lysates of cell lines HeLa, HepG2, and K562 (calculated MW of 57.3 kDa according to NP_002121.4). Recommended concentration: 1-3 μg/mL.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Storage
- -20 °C
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Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))
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Gene ID
- 3157
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NCBI Accession
- NP_002121
Target
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