Insulin-Degrading Enzyme (IDE) (Internal Region) peptide (Biotin)
IDE
Reactivity: Human
Host: Synthetic
BP, Imm
-
- Target See all IDE products
- IDE (Insulin-Degrading Enzyme (IDE))
- Protein Region
- Internal Region
- Origin
- Human
-
Source
- Synthetic
- Purification tag / Conjugate
- This IDE peptide is labelled with Biotin.
- Application
- Blocking Peptide (BP), Immunogen (Imm)
- Sequence
- CQQYNFDRDN TE.
- Predicted Reactivity
- Human, Dog
-
-
- Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 120 kDa band observed in lysates of cell line K562 (calculated MW of 118 kDa according to NP_004960.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
- Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
- Storage
- -20 °C
- Storage Comment
- Shipped at ambient temperature, store at -20°C.
-
- Target
- IDE (Insulin-Degrading Enzyme (IDE))
- Gene ID
- 3416
- NCBI Accession
- NP_004960, NP_001159418, NP_001309722, NP_001309723, NP_001309724, NP_001309726
-
You are here:
