Replication Protein A2, 32kDa (RPA2) (N-Term) Peptide
RPA2
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN5673492
$160.46
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100 μg
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Quick Overview for Replication Protein A2, 32kDa (RPA2) (N-Term) Peptide (ABIN5673492)
Target
RPA2
(Replication Protein A2, 32kDa (RPA2))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- N-Term
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Sequence
- MWNSGFESYG SSSYGGAGGY TQSPGGFGSP APSQAEKKSR ARAQHIVPCT
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Characteristics
- This is a synthetic peptide designed for use in combination with anti- RPA2 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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Background
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As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Alias Symbols: REPA2, RPA32, RP-A p32, RP-A p34
Protein Size: 278 -
Gene ID
- 6118
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NCBI Accession
- NM_001286076, NP_001273005
Target
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