V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (C-Term) Peptide
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- Target See all CrkL (CRKL) products
- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
- Protein Region
- C-Term
- Origin
- Human, Mouse, Rat
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Source
- Synthetic
- Application
- Blocking Peptide (BP), BioImaging (BI)
- Characteristics
- CRKL peptide corresponds to 12 amino acids near the C-terminus region of CRKL.
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- Application Notes
- CRKL Peptide is used for blocking the activity of CRKL antibody . The CRKL antibody ships at 0.5 mg/mL and the CRKL Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- CRKL peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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