ATX-II Peptide
Reactivity: Anemonia sulcata
Host: Synthetic
BP
1 image
Catalog No. ABIN7582137
Quick Overview for ATX-II Peptide (ABIN7582137)
Target
ATX-II
Origin
Anemonia sulcata
Source
Synthetic
Application
Blocking Peptide (BP)
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Peptide Type
- Synthetic
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Purpose
- A Modulator of NaV Channels
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Sequence
- GVPCLCDSDG PSVRGNTLSG IIWLAGCPSG WHNCKKHGPT IGWCCKQ-OH
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Specificity
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Toxin target: Various NaV channels,
Disulfide bonds between: Cys4-Cys44, Cys6-Cys34 and Cys27-Cys45 -
Characteristics
- A Modulator of NaV Channels
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Purity
- ≥98 % (HPLC)
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ATX-II peptide (Atto 647N)
Reactivity: Anemonia sulcata Host: Synthetic BP, FACS, FM, IF, LCI
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Application Notes
- Antigen Preadsorption Control: 10 - 100 nM
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Comment
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Electrophysiology
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- ATX-II
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Background
- Neurotoxin 2, Toxin ATX-II, As2, Anemonia viridis toxin 2, Av2, Delta-actitoxin-Avd1c 2, Toxin II,ATX-II is a 47 amino acid peptidyl toxin, originally isolated from Anemonia sulcata sea anemone venoM It is a potent neurotoxin, which modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes.ATX-II has been used as a powerful activator of TTX-sensitive and -insensitive Na+ channels in various excitable tissue and cell types (at concentration range of 10-100 nM).1-9
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Molecular Weight
- 4935 Da
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UniProt
- P01528
Target
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