ATX-II peptide (Atto 647N)
Reactivity: Anemonia sulcata
Host: Synthetic
FACS, FM, IF, LCI
Catalog No. ABIN7582138
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Quick Overview for ATX-II peptide (Atto 647N) (ABIN7582138)
Target
ATX-II
Origin
Anemonia sulcata
Source
Synthetic
Application
Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Biological Activity
- Active
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Purification tag / Conjugate
- Atto 647N
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Purpose
- ATX-II-ATTO Fluor-647N
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Sequence
- GVPCLCDSDG PSVRGNTLSG IIWLAGCPSG WHNCKKHGPT IGWCCKQ-OH
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Specificity
- Toxin target: NaV1.1-1.7 voltage-gated sodium channels
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Characteristics
- ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is 1-2 Molecules of dye per ATX-II molecule, linked via primary amines.
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Biological Activity Comment
- ATX-II potently modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX-II has been used as a powerful activator of TTX-sensitive and insensitive Na+ channels in various excitable tissue and cell types1-9.
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ATX-II peptide
Reactivity: Anemonia sulcata Host: Synthetic
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Application Notes
- Antigen Preadsorption Control: 0.1 - 1 μM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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Handling Advice
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light
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Storage
- -20 °C
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Storage Comment
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
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- ATX-II
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Background
- A fluorescent probe for imaging and sensitive detection of NaV sodium channels
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Molecular Weight
- 5561 Da
Target
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