MmTx1 Toxin peptide (Atto 488)
Reactivity: Micrurus mipartitus
Host: Synthetic
FACS, IF, LCI
Catalog No. ABIN7582257
$568.46
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Quick Overview for MmTx1 Toxin peptide (Atto 488) (ABIN7582257)
Target
MmTx1 Toxin
Origin
Micrurus mipartitus
Source
Synthetic
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Biological Activity
- Active
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Purification tag / Conjugate
- Atto 488
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Purpose
- MmTx1 Toxin-ATTO Fluor-488
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Sequence
- LTCKTCPFTT CPNSESCPGG QSICYQRKWE EHRGERIERR CVANCPAFGS HDTSLLCCTR DNCN-OH
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Specificity
- Toxin target: GABA(A) receptors
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Characteristics
- ATTO 488 (Ex/Em: 500/520 nm). The extent of labeling is one molecule of dye per MmTx1 Toxin molecule, linked via primary amines.
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Biological Activity Comment
- MmTx1 is a potent allosteric modulator of GABA(A) receptors1.
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Application Notes
- Antigen Preadsorption Control: 200 - 400 nM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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Handling Advice
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light
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Storage
- -20 °C
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Storage Comment
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
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- MmTx1 Toxin
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Background
- A fluorescent probe for imaging and sensitive detection of GABA(A) receptors
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Molecular Weight
- ~7775 Da
Target
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