omega-agatoxin IVA peptide (Biotin)
Reactivity: Agelenopsis aperta
Host: Synthetic
FACS, FM, IF, LCI
Catalog No. ABIN7887370
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Quick Overview for omega-agatoxin IVA peptide (Biotin) (ABIN7887370)
Target
omega-agatoxin IVA
Origin
Agelenopsis aperta
Source
Synthetic
Application
Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Biological Activity
- Active
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Purification tag / Conjugate
- Biotin
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Purpose
- ω-Agatoxin IVA-Biotin
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Sequence
- KKKCIAKDYG RCKWGGTPCC RGRGCICSIM GTNCECKPRL IMEGLGLA-OH
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Specificity
- Toxin target: P/Q-type calcium channels (CaV2.1)
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Characteristics
- LC-Biotin. The extent of labeling is 1-2 Molecules of biotin per ω-Agatoxin IVA molecule, linked via primary amines.
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Biological Activity Comment
- ω-Agatoxin IVA is a blocker of voltage-sensitive P-type Ca2+ channels1. It blocks neuromuscular transmission presynaptically in a variety of synapses2,3.
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omega-agatoxin IVA peptide (Atto 647N)
Reactivity: Agelenopsis aperta Host: Synthetic FACS, FM, IF, LCI
omega-agatoxin IVA peptideReactivity: Agelenopsis aperta Host: Synthetic
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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Handling Advice
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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Storage
- -20 °C
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Storage Comment
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- omega-agatoxin IVA
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Background
- A biotinylated ω-Agatoxin IVA for imaging and sensitive detection of P/Q-Type CaV Channels and Presynaptic Neuromuscular Junctions.
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Molecular Weight
- 5710 Da
Target
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