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DkTx peptide (Atto 647N)

Reactivity: Cyriopagopus schmidti Host: Synthetic FACS, FM, IF, LCI

Representative time course of TRPV1 channel current activation by a continuous application of 2 μM DkTx-ATTO (ABIN7885645), duration indicated by bar (bottom right), compared to activation by 1 μM Capsaicin (ABIN7885724), at a holding potential of -60 mV. (DkTx peptide (Atto 647N))

Dorsal root ganglia (DRG) cells were isolated from the lumbar and thoracic regions of 3-week-old Sprague-Dawley rats and cultured in DMEM/F12 medium supplemented with 100 ng/mL Native mouse NGF 2. (DkTx peptide (Atto 647N))

Dorsal root ganglia (DRG) cells were isolated from the lumbar and thoracic regions of 3-week-old Sprague-Dawley rats and cultured in DMEM/F12 medium supplemented with 100 ng/mL Native mouse NGF 2. (DkTx peptide (Atto 647N))

A. (DkTx peptide (Atto 647N))

A. (DkTx peptide (Atto 647N))

cells. (DkTx peptide (Atto 647N))

A. (DkTx peptide (Atto 647N))

A. (DkTx peptide (Atto 647N))

8 images

Catalog No. ABIN7885645

$1,142.77

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Quick Overview for DkTx peptide (Atto 647N) (ABIN7885645)

Target

DkTx

Origin

Cyriopagopus schmidti

Source

Synthetic

Application

Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)

Peptide Type

Synthetic

Biological Activity

Active

Purification tag / Conjugate

Atto 647N

Purpose

DkTx-ATTO Fluor-647N

Sequence

DCAKEGEVCS WGKKCCDLDN FYCPMEFIPH CKKYKPYVPV TTNCAKEGEV CGWGSKCCHG LDCPLAFIPY CEKYR-OH

Specificity

Toxin target: TRPV1 channel

Characteristics

ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is one molecule of dye per one DkTx molecule, linked via primary amines.

Biological Activity Comment

Selectively activates the heat-activated TRPV1 channel1,2.
Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.

Buffer

Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice

Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light

Storage

-20 °C

Storage Comment

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
Target

DkTx

Background

A fluorescent probe for imaging and sensitive detection of TRPV1 Channel

Molecular Weight

9155 Da

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