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- Target
- DnaK
- Peptide Type
- Recombinant
- Protein Region
- AA 385-546
- Origin
- All Species
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST
- Characteristics
-
Purified recombinant E.coli Dnak protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
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DnaK (AA 385-638), (C-Term) peptide
Reactivity: All Species Host: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
DnaK (AA 508-638) peptideReactivity: All Species Host: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
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- Application Notes
- Dnak protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 25 mM Tris-HCl, pH7.5, containing 2 mM beta-mercaptoethanol, and 1 mM EDTA.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- DnaK
- Background
-
DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
Alternative Names: Heat shock protein 70 protein, Chaperone Hsp70 protein, dnaK protein, seg protein, grpF protein, Heat shock 70 kDa protein protein, , Chaperone protein dnaK protein, Co chaperone with DnaJ protein, groP protein, HSP70 protein - Molecular Weight
- 17.7 kDa (163 AA)
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