DnaK (AA 385-638), (C-Term) Peptide
Reactivity: All Species
Host: Escherichia coli (E. coli)
SDS
Dithiothreitol (DTT)
1 image
Catalog No. ABIN934891
Quick Overview for DnaK (AA 385-638), (C-Term) Peptide (ABIN934891)
Target
DnaK
Origin
All Species
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Peptide Type
- Recombinant
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Protein Region
- AA 385-638, C-Term
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Sequence
- MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HSTRKQVEEA GDKLPADDKT AIESALTALE TALKGEDKAA IEAKMQELAQ VSQKLMEIAQ QQHAQQQTAG ADASANNAKD DDVVDAEFEE VKDKK
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Characteristics
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Purified recombinant E.coli Dnak protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 95 % pure
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DnaK (AA 385-546) peptide
Reactivity: All Species Host: Escherichia coli (E. coli) SDS
DnaK (AA 508-638) peptideReactivity: All Species Host: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
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Application Notes
- Dnak protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 25 mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 5 mM DTT, and 10 % glycerol.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- DnaK
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Background
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DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
Alternative Names: Heat shock 70 kDa protein protein, Chaperone protein dnaK protein, dnaK protein, Co chaperone with DnaJ protein, Dnak (C-term protein, , Heat shock protein 70 protein, Substrate binding domain protein, Chaperone Hsp 70 protein, HSP70 protein, Heat shock 70 kDa protein protein, 385-638) protein -
Molecular Weight
- 27.7 kDa (255 AA)
Target
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