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- Target
- DsbE
- Peptide Type
- Recombinant
- Protein Region
- AA 26-185
- Origin
- E. coli
- Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q
- Characteristics
-
Purified recombinant E.coli DsbE protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
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- Application Notes
- DsbE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM EDTA, and 10 % glycerol.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- DsbE
- Background
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DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: yejQ protein, Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG..ECK2187 protein, Periplasmic thioredoxin of cytochrome c type biogenesis protein, CcmG protein, JW2183. - Molecular Weight
- 18.1 kDa (161 AA)
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