Deoxynucleotidyltransferase, terminal (DNTT) (Middle Region) Peptide
DNTT
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN975940
Quick Overview for Deoxynucleotidyltransferase, terminal (DNTT) (Middle Region) Peptide (ABIN975940)
Target
TdT (DNTT)
(Deoxynucleotidyltransferase, terminal (DNTT))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-DNTT antibody (Catalog #: ARP52032_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))
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Background
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DNTT is the template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Alias Symbols: TDT
Protein Interaction Partner: DNTTIP1,DNTTIP2,PCNA,PRPF19,XRCC6,ABTB2,CUL3,DNTTIP2,PCNA,PRPF19,UBC,XRCC6
Protein Size: 509 -
Molecular Weight
- 58 kDa
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Gene ID
- 1791
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NCBI Accession
- NM_004088, NP_004079
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UniProt
- P04053
Target
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