Fat Mass and Obesity-Associated (FTO) (N-Term) Peptide
FTO
Reactivity: Mouse
Host: Synthetic
BP, WB
Catalog No. ABIN977128
Quick Overview for Fat Mass and Obesity-Associated (FTO) (N-Term) Peptide (ABIN977128)
Target
FTO
(Fat Mass and Obesity-Associated (FTO))
Origin
Mouse
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- N-Term
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Sequence
- MKRVQTAEER EREAKKLRLL EELEDTWLPY LTPKDDEFYQ QWQLKYPKLV
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-Fto Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- FTO (Fat Mass and Obesity-Associated (FTO))
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Background
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Fto is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Fto has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Fto has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Fto has no activity towards 1-methylguanine and no detectable activity towards double-stranded DNA. Fto requires molecular oxygen, alpha-ketoglutarate and iron. Fto contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Fto contributes to the regulation of body size and body fat accumulation.
Alias Symbols: AW743446, mKIAA1752
Protein Size: 502 -
Molecular Weight
- 58 kDa
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Gene ID
- 26383
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NCBI Accession
- NM_011936, NP_036066
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UniProt
- Q8BGW1
Target
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