Ubiquitin Protein Ligase E3A (ube3a) (Middle Region) Peptide
ube3a
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN985848
$160.46
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100 μg
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Quick Overview for Ubiquitin Protein Ligase E3A (ube3a) (Middle Region) Peptide (ABIN985848)
Target
ube3a
(Ubiquitin Protein Ligase E3A (ube3a))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-UBE3A antibody (Catalog #: ARP42951_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
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Background
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UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
Alias Symbols: ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A
Protein Interaction Partner: UBE2L3,UBE2L6,AR,BLK,BPY2B,BPY2C,BPY2C,EAPP,MCM7,PGR,PHF17,RARA,SCAMP1,SHBG,SMAD9,TP53,UBE2D1,UBE2D3,UBE2E1,UBE2G1,UBE2G2,UBE2L3,UBE2L6,UBE3A,UBQLN2,BARD1,BLK,BPY2,BRCA1,ESR2,LCK,MCM7,PGR,TSC2,UBE2D1,UBE2D2,UBE2D3,UBE2L3,UBQLN1,UBQLN2
Protein Size: 875 -
Molecular Weight
- 101 kDa
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Gene ID
- 7337
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NCBI Accession
- NM_000462, NP_000453
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UniProt
- Q05086
Target
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