CNTF Protein (AA 4-196, partial) (His tag)

Catalog No. ABIN1095544

Recombinant CNTF protein expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.

Quick Overview for CNTF Protein (AA 4-196, partial) (His tag) (ABIN1095544)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 4-196, partial

Purification tag / Conjugate: This CNTF protein is labelled with His tag.

Sequence: TEHSPLTPHR RDLCSRSIWL ARKIRSDLTA LTESYVKHQG LNKNINLDSA DGMPVASTDQ WSELTEAERL QENLQAYRTF HVLLARLLED QQVHFTPTEG DFHQAIHTLL LQVAAFAYQI EELMILLEYK IPRNEADGMP INVGDGGLFE KKLWGLKVLQ ELSQWTVRSI HDLRFISSHQ TGIPARGSHY IAN

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Negro, Tolosano, Skaper, Martini, Callegaro, Silengo, Fiorini, Altruda: "Cloning and expression of human ciliary neurotrophic factor." in: European journal of biochemistry / FEBS, Vol. 201, Issue 1, pp. 289-94, (1991) (PubMed).

Masiakowski, Liu, Radziejewski, Lottspeich, Oberthuer, Wong, Lindsay, Furth, Panayotatos: "Recombinant human and rat ciliary neurotrophic factors." in: Journal of neurochemistry, Vol. 57, Issue 3, pp. 1003-12, (1991) (PubMed).

Lam, Fuller, Miller, Kloss, Manthorpe, Varon, Cordell: "Sequence and structural organization of the human gene encoding ciliary neurotrophic factor." in: Gene, Vol. 102, Issue 2, pp. 271-6, (1991) (PubMed).

Target Details

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: Ciliary neurotrophic factor protein

Background: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Molecular Weight: 26.2 kD

UniProt: P26441

Pathways: JAK-STAT Signaling