DDOST Protein (AA 44-427, partial) (GST tag)

Catalog No. ABIN1046935

This Recombinant DDOST protein is expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.

Quick Overview for DDOST Protein (AA 44-427, partial) (GST tag) (ABIN1046935)

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 44-427, partial

Purification tag / Conjugate: This DDOST protein is labelled with GST tag.

Sequence: GPRTLVLLDN LNVRETHSLF FRSLKDRGFE LTFKTADDPS LSLIKYGEFL YDNLIIFSPS VEDFGGNINV ETISAFIDGG GSVLVAASSD IGDPLRELGS ECGIEFDEEK TAVIDHHNYD ISDLGQHTLI VADTENLLKA PTIVGKSSLN PILFRGVGMV ADPDNPLVLD ILTGSSTSYS FFPDKPITQY PHAVGKNTLL IAGLQARNNA RVIFSGSLDF FSDSFFNSAV QKAAPGSQRY SQTGNYELAV ALSRWVFKEE GVLRVGPVSH HRVGETAPPN AYTVTDLVEY SIVIQQLSNG KWVPFDGDDI QLEFVRIDPF VRTFLKKKGG KYSVQFKLPD VYGVFQFKVD YNRLGYTHLY SSTQVSVRPL QHTQYERFIP SAYP

Characteristics: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Application Details

Comment:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS buffer, 20mM GSH

Storage: -20 °C

References

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, (2003) (PubMed).

Yamagata, Tsuru, Momoi, Suwa, Nozaki, Mukasa, Ohashi, Fukushima, Momoi: "Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)." in: Genomics, Vol. 45, Issue 3, pp. 535-40, (1998) (PubMed).

Nagase, Miyajima, Tanaka, Sazuka, Seki, Sato, Tabata, Ishikawa, Kawarabayasi, Kotani: "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 2, Issue 1, pp. 37-43, (1995) (PubMed).

Target Details

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit

Background: Synonyms: DDOST 48 kDa subunit,Oligosaccharyl transferase 48 kDa subunit

Molecular Weight: 70 kD

UniProt: P04843

Pathways: Cell RedoxHomeostasis